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The quality of a bioproduction is assessed by attributes such as the amount of recombinant proteins produced. Optimisation of a bioprocess requires numerous protein quantifications and would highly benefit from continuous monitoring of production and downstream processing steps.

Here a novel approach based on fluorescent polarization and specific fluorescent probes was developed. The new quantification assay is rapid (typically less than 20 min.) and easy-to-use as it only requires mixing of a small amount of the fermentation or cell culture with fluorescent reagents. Typical limits of detection were 0.3 mg/ml for His-tagged proteins and 30 mg/ml for antibodies. Qualitative and quantitative monitoring of the production of various proteins during fermentations, respectively mammalian cell cultures, was performed « at-line ». For target proteins localized in bacterias, detergents were added to release the product. The assay offered in addition the possibility to monitor « at-line » the affinity purification of the recombinant proteins for quick determination of the product-containing fractions. Further developments of this rapid and easy-to-use quantification assay could allow online monitoring of production and downstream processing.